Undetectable Alanine Aminotransferase during Hospitalization

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Alanine Aminotransferase

An examination of the subcellular distribution of alanine aminotransferase activity in pig cardiac tissue showed that about 10% of the total activity was bound to particulate material, with the highest specific activity in the sarcosomal fraction. The soluble enzyme was obtained in a high state of purity, as indicated by sedimentation velocity, starch gel electrophoresis, and spectral analyses....

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Optimization of methods for aspartate aminotransferase and alanine aminotransferase.

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Affinity Labeling of Alanine Aminotransferase by 3Xhloro+alanine*

The pyridoxal form of alanine aminotransferase from pig heart catalyzes the a,/? elimination reaction with 3chloro-L-alanine as the substrate to form equimolar amounts of pyruvate, ammonia, and chloride. The maximum rate of the a,/3 elimination reaction was 2.5 pmol/min/mg at pH 7.0 (25”(Z), approximately 0.5% that of the transamination reaction between L-alanine and 2-oxoglutarate. Time-depend...

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ژورنال

عنوان ژورنال: Clinical Chemistry

سال: 2016

ISSN: 0009-9147,1530-8561

DOI: 10.1373/clinchem.2015.247692